超高效液相色谱-串联质谱法同时测定浓缩果汁中的18种多酚物质

摘要

A UPLC - MS/MS method was developed for the determination of 18 polyphenolic compounds in juice concentrate. The sample was diluted with water and extracted completely by an Oasis (HLB) SPE column. The extract was evaporated to dryness and redissolved with methanol -0. 1% formic acid. The targeted compounds were separated on an Acquity UPLC? BEH Clg( 1. 7 im x 2.1 mm ×50 mm) column using 0. 1% formic acid - methanol as mobile phase by linear gradient elution at a flow rate of 0. 3 mL ? Min-1, and detected by MS/MS. The quantification of eighteen polyphenolic compounds was performed by the external standard method. Under the optimal conditions, the calibration curves of 18 polyphenolic compounds were linear in the range of 10. 0 - 10 000. 0 μg ? L-1 with detection limits of 10. 0 - 1 000. 0 ng ? G-1. The average recoveries for all the analytes were more than 70% with RSDs ( n = 6 ) less than 8. 0% . With the advantages of good reproducibility, good recovery and high sensitivity, the method was suitable for the analysis of polyphenolic compounds in juice concentrate.%建立了浓缩果汁中18种多酚物质的超高效液相色谱(UPLC)-串联质谱(MS/MS)检测方法.样品经水稀释,HLB固相萃取净化,浓缩蒸干后用甲醇和0.1%甲酸定容.采用Acquity UPLC BEH C18 (1.7μm ×2.1 mm ×50 mm)色谱柱分离,以甲醇和0.1%甲酸为流动相,在0.3 ml,·min-1流速下梯度洗脱,MS/MS进行检测、外标法定量.果汁中18种多酚在10.0-10 000.0 μg· L-1范围内线性良好(r > 0.990 0),检出限(LOD)为10.0-1000.0ng·g-1,平均加标回收率在70%以上,相对标准偏差(RSD,n=6)均小于8.0%.方法具有杂质干扰小、回收率高、重复性好、灵敏度高等特点,适用于果汁中多酚物质的定性定量分析.