Determination of Quercetin and its metabolites in rat plasma by ultra high performance liquid chromatography tandem mass spectrometry

摘要

Fast, selective and reliable method was developed, optimized and validated for determination of quercetin and its metabolites by UHPLC-MS/MS. Protein precipitation was optimized as a sample preparation step for rat plasma. The method was used for pharmacokinetic studies and for the explanation of metabolism and possible cardioprotective effect of quercetin and its metabolites in vivo.