With their high chiral recognition to a broad range of chiral compounds, polysaccharide derivatives-based chiral stationary phases (CSPs) have been regarded as the most powerful CSPs in the direct resolution of enantiomers by HPLC. Since there are no chemical linkages between the polysaccharide derivatives and the supports, the coating - type CSPs exhibit some disadvantages such like poor solvent durability and rather limited mobile phase selection. With broadened mobile phase selection and improved stability, immobilizing-type polysaccharide-based CSPs overcome the disadvantages of the coated ones, and have become the hot topics in chiral separation in recent years. In this paper, five immobilization methods of the polysaccharide-based CSPs, including the bifunctional reagent method, chemical bonding of the amylose derivative via an activated chain end, radical polymerization, photoirradiation and intermolecular polycondensation, were introduced and compared, Furthermore, the further developments of those CSPs are prospected.%多糖类衍生物手性固定相因其强大的手性分离能力,在液相色谱直接拆分对映体方面应用广泛.涂敷型手性固定相因多糖衍生物与基质间无化学键,因此溶剂耐受性差,流动相的选择十分有限.而键合型多糖类手性固定相克服了涂敷型手性固定相的缺点,扩大了流动相的选择范围,提高了手性固定相的稳定性,成为近年来手性分离领域的研究热点.该文对这类固定相的5种制备方法:双官能团试剂法、端基还原法、共聚法、光化学法以及分子间缩聚法进行了介绍,对各种制备方法的优缺点进行了比较,并对其发展方向进行了展望.
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