Based on the polyaniline nano fiber(PANI-NF)-nanometer gold composite film( AuNPs) constructing electrode interface and then modified by transferrin, HeLa cells can be captured on the modified electrode due to the specific identification function by transferrin and transferrin receptor, resulting in the variation of electrochemical impedance values. The effects of drug ( curcumin meat) on HeLa cells were investigated by ectrochemical impedance spectroscopy. The results showed that with the increase of drug concentration and prolongation of function time, electrochemical impedance values were decreased, indicating that the inhibition effects of drug (curcumin) on HeLa cells were enhanced. A quantitative relation between the change rate of electrochemical impedance values, drug concentration and treating time was observed. The results of electrochemical experiment are in accordance with that of the MTT method and inverted microscope. Based on this, a new method for testing the inhibition rate of drug on cells was established. The method is simple, high sensitivity and low cost.%以聚苯胺纳米纤维(PANI-NF) -纳米金(AuNPs)复合膜构建传感界面,在AuNPs/PANI-NF界面上修饰转铁蛋白(Tf),利用转铁蛋白与人宫颈癌细胞(HeLa)表面大量表达的转铁蛋白受体(TfR)之间的特异性识别作用,将细胞捕获到传感器界面,导致电化学阻抗值变化.利用电化学阻抗谱研究姜黄素对HeLa细胞的抑制作用.结果表明,随着药物浓度的增大和作用时间的延长,电化学阻抗值下降,表明姜黄素对细胞的抑制作用增强.电化学阻抗值的变化率与药物浓度和作用时间具有量效关系；电化学方法检测显示,姜黄素对HeLa细胞的抑制作用趋势与MTT法及倒置显微镜检测到的姜黄素对HeLa细胞的抑制作用结果相吻合,从而建立了一种检测药物对细胞抑制作用的新方法.该方法具有简便、灵敏度高、费用低廉等优点.
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