内燃机油高效液相指纹图谱的建立

摘要

建立了内燃机油指纹图谱的高效液相色谱(HPLC)分析方法.采用反相C18柱,以乙腈-水为流动相梯度洗脱,检测波长280 nm,柱温30℃,进样体积10 μL进行试验.考察结果表明,指纹图谱中各共有色谱峰的相对保留时间α和相对峰面积Sr的相对标准偏差(RSD)均小于2.4％,满足小于3％的指纹图谱研究技术要求.通过共有特征峰分析以及色谱峰重叠率、相似度的计算,可进一步鉴别和评价内燃机油的质量.该法与传统的内燃机油品质评价方法相比,节约了试验成本和时间,为内燃机油的快速鉴别和质量控制开拓了新领域.%A method of chromatography fingerprint of engine oil by HPLC was established.The chromatographic conditions were as follows:RP-C18 column,mobile phase:acetonitrile-water by gradient elution,detection wavelength:280 nm,column temperature:30 ℃,injection volume:10 μL.The methodological evaluation showed that the relative standard deviations(RSDs) of relative time (α) and relative area of chromatographic peaks (Sr) from common peaks were all less than 2.4％,which could meet the technical requirement of the fingerprint(less than 3％).By further analyzing the main characteristic peaks and computing the chromatographic peaks overlapping ratios and the similarity,could be identified and evaluated the quality of engine oil.Compared with the traditional methods,this method greatly reduced the cost of trial and time,and provided a new area for the application of rapid identification and quality control of engine oils.