银纳米粒子增强变色酸2R的荧光及应用于BSA检测

摘要

Under acidic conditions,the flourescence of CT2R could be enhanced with silver nanop-articles because of its surface enhancement effect. This enhancement effect would be decreased in the presence of BSA. The decreased fluorescence intensity is proportional to the concentration of BSA. Ac-cording to this principle,a simple and new method for BSA determination was developed. The influences of some experimental factors,including pH value,concentration of CT2R,concentration of silver nanop-articles,addition order of reagents on the determination of BSA were investigated. Under the optimal con-ditions,the linear range for BSA detection is 0. 02-1. 00 mg/L,with a detection limit of 0. 002 6 mg/L. The method was used in the detection of BSA in synthetic samples with satisfactory results.%利用酸性条件下，牛血清白蛋白（ BSA）可降低银纳米粒子-变色酸2R（ CT2R）体系的表面增强荧光效应，建立了一种测定BSA的荧光分析新方法。考察了pH值、CT2R的浓度、银纳米粒子浓度、试剂加入顺序和共存物质等因素对测定BSA的影响。实验结果表明，在pH值为5.72，CT2R的浓度为1.5×10-5 mol/L，银纳米粒子浓度（以银原子计算）为1.25×10-4 mol/L，按银纳米粒子、BSA、CT2R、BR缓冲溶液依次添加的条件下，BSA的线性范围为0.02~1.00 mg/L，检出限为0.0026 mg/L。该法用于合成样品中BSA的测定，灵敏度高，重现性好，结果准确。