直接稀释/电感耦合等离子体质谱法测定人血清中微量元素

摘要

建立了一种高灵敏度、高通量和低样品消耗的人血清中微量元素的质谱检测方法.针对血清样品基质复杂的特点,采用正四丁基氢氧化铵、曲拉通和EDTA的混合液直接稀释样品,使用内标元素校正和碰撞反应池技术来抑制基质效应和多原子离子的干扰,通过仪器调谐和碰撞反应池工作参数的优化,成功检测了人体血清中Al、Cu、Fe、Zn、Ga、As、Cd、Hg、Tl、Pb 10种微量元素的含量,各元素的加标回收率为87.2％ ～ 111.2％.方法用于人血清标准物质(ClinChek(R)-Control,Level Ⅰ and Level Ⅱ)测试,测定值与标准值相符.采用此方法对127名成年健康人血清样品进行检测,基于检测结果给出了不同年龄、性别组的均值和测量值范围.%A method with high sensitivity,high throughout and low sample consumption was developed for the analysis of trace elements in human serum by inductively coupled plasma mass spectrometry(ICP-MS).As the matrix was complicated,the sample was diluted with a mixture of four butyl ammonium hydroxide,Triton and EDTA.The internal standard correlation and collision/reaction cell were used to eliminate the matrix effects and polyatomic ion inteferences.Ten trace elements of Al,Cu,Fe,Zn,Ga,As,Cd,Hg,Tl and Pb were simultaneously determined after tuning up the instrument and optimizing the collision/reacion cell parameters,and the spiked recovery for each element ranged from 87.2％ to 111.2％.This method was used in the analysis of human serum standard (ClinChek(R)-Control,Level Ⅰ and Level Ⅱ).The determined values were in good agreement with the certified values.The reference values for different age groups and sex groups were listed based on the determination of 127 serum samples from healthy adults.