Determination of cystatin C in human serum by isotope dilution mass spectrometry using mass overlapping peptides

摘要

The combination of MOPs, decreased resolution in the first mass analyzer and multiple linear regression tries to improve the current accuracy and precision of absolute quantifications of low levels of proteins in real samples. The procedure is able to measure precise and accurate isotope distributions and can be applied for the measurement of low μg g-1 levels of Cystatin C in serum samples with satisfactory results.