利用氯化钙-乙醇-水(n(CaCl2):n(EtOH):n(H2O)=1:2:8)三元溶液对丝素蛋白纤维预处理,再采用交替浸渍法将其分别浸在钙溶液和磷溶液中交替浸渍、仿生矿化制备丝素蛋白纤维/磷灰石复合材料,采用SEM、FTIR、XRD和TGA等技术研究了不同预处理时间对丝素蛋白纤维微结构和构建丝素蛋白纤维/磷灰石的影响.结果表明,预处理增加了丝素蛋白纤维表面粗糙程度及内部孔隙率,并使钙离子与丝素蛋白纤维中的羧基、羟基和酰胺基等基团先发生作用,可以有效地提高磷灰石在丝素蛋白表面的沉积量,使得预处理后的丝素蛋白纤维与磷灰石的结合更均匀、密实,获得了磷灰石晶体沿c轴取向生长的仿骨结构的复合材料.%To develop biomimetic fibroin/apatite composites with bone-like structure by alternate soaking method,the degummed natural fibroin fibers were pretreated by soaking into CaCl2：EtOH：H2O (n(CaCl2)：n(EtOH)：n(H2O)= 12：8) solution for given time and then soaked into calcium solution and phosphate solution, respectively, to fabricate fib roin/apatite composites. The effects of pretreatment time and the alternate soaking times on fibroin microstructure and fibroin/apatite composites were examined by SEM, FTIR, XRD and TGA techniques. The pretreatment using CaCl2：EtOH：H2O(n(CaCl2)：n(EtOH)：n(H2O)= 1:2:8) solution makes fibroin fiber exhibit rougher surface and higher internal porosity, which is beneficial to interaction of calcium ion and residue groups such as -COOH, -OH and -CONH- from fibroin, and then improve the deposition of apatite on fibroin fibers. The homogeneous apatites with oriented c-axis were formed on the pretreated fibroin fibers. Moreover, there are more than 30wt％ apatites deposited on fibroin fibers after altemate soaking for more than 3 times. The fibroin/apatite composite has potential to be applied as bone repair materials.
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