目的：：探讨β-谷甾醇在抵抗阿司匹林胃黏膜损伤剂量下，是否影响阿司匹林的抗炎药理作用，为研制可降低阿司匹林胃黏膜损伤副作用的复方制剂提供药理研究基础。方法：将SD大鼠、昆明种小鼠各40只，分别随机分为4组：阿司匹林组、阿司匹林+β-谷甾醇组、模型组与空白组，各组给药7d后，用角叉菜胶(卡拉胶)诱导大鼠足趾肿胀模型，用皮尺测量大鼠足周长，计算肿胀度；用二甲苯诱导小鼠耳肿胀模型，测量各组小鼠左右耳重量，计算肿胀度。结果：(1)各组大鼠足趾肿胀度与空白组相比，差异具有显著性(P<0.05)，给药组大鼠足趾肿胀度与模型组相比，差异具有显著性(P<0.05)，谷甾醇+阿司匹林组与阿司匹林组大鼠足趾肿胀度相比，差异具有显著性(P<0.05)；(2)各组小鼠耳肿胀度与空白组相比，差异具有显著性(P<0.05)，给药组小鼠耳肿胀度与模型组相比，差异具有显著性(P<0.05)，谷甾醇+阿司匹林组与阿司匹林组小鼠耳肿胀度相比，差异具有显著性(P<0.05)。结论：实验结果表明：(1)成功复制了卡拉胶诱导的大鼠足趾肿胀的炎症模型，成功复制了二甲苯诱导的小鼠耳肿胀的炎症模型；(2)通过大鼠足趾肿胀与小鼠耳肿胀炎症模型证实，阿司匹林与β-谷甾醇联合使用比单独使用阿司匹林的抗炎效果更强。%Objective:This study investigated influence of beta-sitosterol on anti-inflammatory effect of aspirin in a relatively effective gastric mucosa protection doses. Methods:40 Rats and 40 mouse were respectively randomly parallelly assigned 4 groups:control group( n=10;CMC-Na) ,model group(n=10),ASPL(n=10;asprilin),or and SASPL(n=10:asprilin plus beta-sitosterol). Each group rats received a subplantar injection of carrageenan(0. 1mL of a 1%suspension in 0. 85%saline) into the right hind paw. Paw volume was measured immediately prior to the injection of carrageenan and thereafter at hourly intervals for 6h. Oedema was expressed as the increase in paw volume aftercarrageenan injection relative to the pre-injection value for each animal. Each group mices;0. 03mL of xylene was applied to the anterior and posterior surfaces of the right ear. The left ear wasconsidered as control. four hours after xylene application,mice were killed and both ears were removed. Circular sections were taken,using a cork borer with a diameter of 6mm,and weighed. The increase in weight caused by the irritant was measured by subtracting the weight of the untreated left ear section from that of the treated right ear sections. Results:There was significant differences between ASPL and SASPL(P<0. 05). Conclusion:Asprilin plus beta-sitosterol significantly enhanced the level of anti-inflammatory effect of aspirin(P<0. 05)by two animal models of inflammatory.
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