水溶性黑柄炭角菌肽的体外抗氧化活性

摘要

Objective To study anti-oxidative activity of water-soluble peptides from Xylaria nigripes (XNP) in vitro.Methods The anti-oxidative activity of water-soluble peptides from XNP were studied entirely by measuring scavenging rate of water-soluble peptides from XNP on super-oxide anion generated by pyrogallol autuxidaliun,hydroxyl free radical in Fenton reaction and DPPH free radical,and their reducing power and inhabiting peroxidation of liposomes ability.Results Water -soluble peptides from XNP could significantly reduce pyrogallol autoxidation rate,had highly scavenging capacity of hydroxyl free radical,and had dose dependent in scavenging DPPH free radical scavenging.Their IC50 were 10,0.49 mg/mL and 16.3 ug/mL respectively.Futher research showed that water-soluble peptides from XNP had certain reducing power increased as concentration increased,they also could inhibit the peroxidation of liposomes.Conclusion Water-soluble peptides from XNP was a good anti-oxidant in vitro test.%目的 研究水溶性黑辆炭角菌肽的体外抗氧化活性.方法 通过测定水溶性黑柄炭角菌肽对邻苯三酚自氧化产生的超氧阴离子、Fenton反应产生的羟自由基、DPPH自由基的清除率,还原能力及抑制脂质体过氧化,全面考察水溶性黑柄炭角菌肽的体外抗氧化能力.结果 水溶性黑柄炭角菌肽能明显降低邻苯三酚自氧化速率,对羟自 由基有极强的清除能力,对DPPH自由基清除率随浓度增加而增加,其IC50分别为10、0.49 mg/mL和16.3 μg/mL;进一步研究表明,黑柄炭角菌肽具有一定的还原力且与浓度呈正比例关系,同时也能抑制脂质体的过氧化反应.结论 水溶性黑柄炭角菌肽在体外具有明显的抗氧化作用.