咳喘穴位敷贴对哮喘大鼠肺组织JAK1、STAT6表达的影响

摘要

Objective To observe the influence of Kechuan acupoint application therapy on the expression of JAK1, STAT6 in the lung tissue of asthmatic rats. Methods The 40 male SD rats were randomly divided into control group, asth-ma model group, acupoint application group and dexamethasone group, 10 rats in each group. Except the control group, bronchial asthma model rats in other groups were sensitized and stimulated by ovalbumin. After succesfully building the asth-ma model rats, was established, the rats were treated by Kechuan acupoint application. The rats in dexamethasone group were intraperitoneally injected with dexamethasone 0.5 mg/(kg·d). The lung tissues were taken after treatment of 14 d. At the end of therapy, the histopathological change in lung tissues was detected by HE staining. The protein expression of JAK1 and STAT6 were analyzed by immunohistochemical staining. The mRNA expression of JAK1 and STAT6 were determined by real time PCR. Results Compared with control group, the lung tissue of asthmatic model group showed a lot of inflammatory cells infiltration, increased airway secretions and disorder alveolar structure. Compared with the asthma model group, the lung tissueof Kechuan acupoint acupoint group and dexamethasone group showed decreased inflammatory cells infiltration, less airway secre-tions and regular alveolar structure. Compared with control group, the protein and mRNA expression of JAK1 and STAT6 in rats of asthma model group increased significantly (P<0.01,P<0.01). Compared with asthma model group, the protein expression of JAK1 and STAT6 (P<0.05 or P<0.01) in rats of Kechuan acupoint application group and dexamethasone group decreased, the mRNA expres-sion of JAK1 and STAT6 mRNA down-regulated (P<0.01). Conclusion Kechuan acupoint application could imporve bronchial and lung tissue inflammation in asthmatic rats, the mechanism may be related to decreasing mRNA and protein expression of JAK1 and STAT6.%目的 观察咳喘穴位敷贴对慢性支气管哮喘大鼠肺组织JAK1、STAT6表达的影响.方法 将40只SD大鼠随机分为对照组、哮喘模型组、咳喘穴位敷贴组、地塞米松组,每组10只.除对照组外,其余各组采用卵清蛋白致敏并激发的方法 制备慢性支气管哮喘大鼠模型.造模成功后,咳喘穴位敷贴组大鼠相应穴位贴敷咳喘穴位敷贴进行干预治疗,地塞米松组大鼠腹腔注射地塞米松磷酸钠注射液0.5 mg/(kg·d).治疗14 d后取大鼠肺组织,HE染色观察肺组织形态学变化,免疫组织化学、Real time PCR检测肺组织JAK1和STAT6蛋白及mRNA的表达水平.结果 与对照组相比,哮喘模型组大鼠肺组织可见大量炎性细胞浸润,气道内有大量分泌物,肺泡结构紊乱;与哮喘模型组相比,咳喘穴位敷贴组和地塞米松组大鼠肺组织炎性细胞浸润减少、气道分泌物减少、肺泡排列规则.与对照组相比,哮喘模型组大鼠气道上皮JAK1、STAT6蛋白和mRNA表达明显升高(P<0.01);与哮喘模型组相比,咳喘穴位敷贴组和地塞米松组大鼠气道上皮JAK1、STAT6蛋白表达下调(P<0.05或P<0.01),JAK1、STAT6 mRNA表达下调(P<0.01).结论 咳喘穴位敷贴能够改善支气管哮喘大鼠支气管及肺组织炎症,其机制可能与降低JAK1、STAT6 mRNA和蛋白表达有关.