“三穴五针法”对过敏性支气管哮喘大鼠肺组织p-ERK1/2蛋白表达的影响

摘要

Objective To explore the effect of "three-acupoint and five-needling method" on the expression level of p-ERK 1/2 protein in lung tissue of rats with allergic bronchial asthma.Methods Forty SPF-grade male SD rats were selected and randomly divided into four groups,including control group (group C),model group (group M),acupuncture group (group A) and blocker group (group PD),with 10 rats in each group.After corresponding intervention was given,cell counting was conducted,and the expression level of p-ERK 1/2 protein in the lung tissue of rats was detected via immunohistochemical method and Western blot method.Results The number of leukocyte and eosinophil in the bronchoalveolar lavage fluid of rats in group A and group PD was smaller than that in group M,but larger than that in group C.It was shown based on the immunohistochemical test and Western blot test that the expression level of p-ERK1/2 protein in the lung tissue of rats in group M was higher than that in group C (P＜0.05),while the expression level of p-ERK1/2 protein in group A and group PD was lower than that in group M (P＜0.05).Conclusion "Threeacupoint and five-needling method" can effectively relieve the inflammatory response of rats with allergic bronchial asthma,and inhibit the expression level of p-ERK 1/2 protein in the lung tissue of rats with asthma.Acupuncture may alleviate the symptom of allergic bronchial asthma through inhibiting the expression level of p-ERK 1/2 protein.%目的:探讨“三穴五针法”对过敏性支气管哮喘大鼠肺组织p-ERK1/2蛋白表达的影响.方法:取SPF级雄性SD大鼠40只,随机分为空白组(C组)、模型组(M组)、针刺组(A组)及阻断剂组(PD组),每组10只,给予相应干预后,进行细胞计数,采用免疫组织化学法及Western blot法检测大鼠肺组织中p-ERK1/2蛋白的表达程度.结果:A组及PD组大鼠肺泡灌洗液中白细胞及嗜酸性粒细胞较M组少而较C组多,免疫组化法及western blot法均显示M组哮喘大鼠肺组织p-ERK1/2蛋白的表达高于C组(P＜0.05),而A组及PD组大鼠肺组织p-ERK1/2蛋白表达低于M组(P＜0.05).结论:“三穴五针法”可明显改善过敏性支气管哮喘大鼠气道炎性反应,抑制哮喘大鼠肺组织p-ERK1/2蛋白的表达,针刺可能通过抑制p-ERK1/2蛋白的表达而达到减轻过敏性支气管哮喘症状的目的.