益肺通络颗粒体外抗结核及止咳止血作用研究

摘要

目的 研究益肺通络颗粒体外抗结核、止血、止咳作用及对非特异免疫功能的影响,为其临床应用提供实验依据.方法 用人型结核分枝杆菌标准菌株(H37Rv)和临床分离的结核分枝杆菌敏感菌株和耐药菌株,接种于含有不同浓度益肺通络颗粒的培养基中,观察结核杆菌的生长情况;应用剪尾法、毛细管法测定小鼠的出血时间、凝血时间,观察益肺通络颗粒的止血作用;用氨水引咳,观察其止咳作用;通过巨噬细胞碳粒廓清试验,观察其对非特异性免疫功能(廓清指数和校正廓清指数)的影响.结果 益肺通络颗粒在高浓度(0.01~0.2 g/mL)时对结核分枝杆菌有显著的抑菌作用,但随着浓度降低,其抑菌效果也显著减弱,浓度减低至0.025 g/mL以下时抑菌作用不明显;与对照组小鼠出血时间(321.2±34.3)s、 凝血时间(135.8±5.1)s比较,益肺通络高剂量组小鼠出血时间(169.5±23.0)s、凝血时间(97.5±3.3)s均显著缩短(P<0.01);与对照组小鼠氨水引咳的潜伏期(36.4±1.9)s、5 min内小鼠的咳嗽次数(45.9±3.1)次比较,益肺通络高剂量组小鼠氨水引咳的潜伏期(54.1±1.4)s显著延长、5 min内小鼠的咳嗽次数(22.7±2.2)次显著减少(P<0.01);且小鼠廓清指数和校正廓清指数较对照组均有显著提高(P<0.01).结论 益肺通络颗粒具有体外抗结核作用,具有止血止咳和增强非特异性免疫功能的作用.%Objective To study the effects of Yifei Tongluo granules on anti tuberculosis and the effect on relieving cough, hemostatic and non-specific immunological function. Methods Human Mycobacterium tuberculosis H37Rv and clinical isolated Mycobacterium tuberculosis sensitive strain and drug-resistant strains were inoculated in culture medium with different concentrations of Yifei Tongluo granules, separately. Growing states of Mycobacterium tuberculosis were observed. The bleeding time was measured by tail breaking test in mice and the coagulation time was measured by capillary method in mice to ob-serve the hemostasis effect of Yifei Tongluo granules. The antitussive effect was observed by ammonia water induced cough method. The effect on the non-specific immunological function (clearance index and corrected clearance index) was ob-served by macrophage carbon particle clearance test. Results Mycobacterium tuberculosis were significantly inhibited by Yifei Tongluo granules with high concentration (0.01~0.2 g/mL), and the antibacterial effect was significantly reduced by decreasing concentration of Yifei Tongluo granules. The antibacterial effect is not obvious when the concentration of Yifei Tonglu-o granules decreased to 0.025 g/mL. Compared with the bleeding time (321.2±34.3) s and the clotting time (135.8±5.1) s in the control group mice, the bleeding time (169.5±23) s and the coagulation time (97.5±3.3) s in high concentration of Yifei Tongluo group mice were significantly decreased (P<0.01). Compared with the incubation period (36.4±1.9) s of ammonia in-duced cough and cough frequency (45.9±3.1) in 5 min in the control group mice,the incubation period (54.1±1.4) s of ammo-nia induced cough was significantly prolonged and the cough frequency (22.7±2.2) in 5min significantly decreased (P<0.01) in the Yifei Tongluo with high dose group mice. Compared with the control group, the clearance index and the corrected clear-ance index were significantly improved in Yifei Tongluo group mice (P<0.01). Conclusion Yifei Tongluo granules have the ef-fects on anti-tuberculosis, relieving cough and hemostasis and enhancing non-specific immunological function in vitro.