清霾饮对细颗粒物PM2.5致大鼠肺损伤的干预作用研究

摘要

目的 研究清霾饮对大气细颗粒物PM2.5所致大鼠肺损伤的干预作用.方法 健康成年SD大鼠48只随机分为3组:生理盐水组、染毒组、清霾饮干预组.采集造模染毒所需浓度37.5 mg/mL的颗粒物悬液对染毒组和清霾饮干预组进行气管滴注,并在染毒后进行灌胃给药.最后一次染毒48 h 后,收集腹主动脉血和支气管肺泡灌洗液,测定支气管肺泡灌洗液(BALF)中的碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)活力及(MDA)含量和血清中的丙二醛(MDA)含量及超氧化物歧化酶(SOD)活力.结果(1)PM2.5染毒后,大鼠BALF中AKP、LDH活力及MDA含量均较生理盐水组升高;清霾饮干预后,大鼠BALF中AKP、LDH 活力及MDA含量较PM2.5染毒组均降低,差异均有统计学意义(P<0.05);(2)PM2.5染毒后,大鼠血清中MDA含量升高,SOD活力较生理盐水组降低;清霾饮干预后,较PM2.5染毒组血清中MDA含量明显降低,SOD活力稍降低,差异均有统计学意义(P<0.05).结论 细颗粒物PM2.5可以导致大鼠肺部炎性损伤,清霾饮对肺部炎性损伤有拮抗作用.%Objective To study the intervention effect of Qingmai Yin on lung injury induced by atmospheric fine particulate matter PM2.5 in rats. Methods The 48 healthy adult SD rats were randomly divided into three groups: normal saline group, exposure group, and Qingmai Yin intervention group. The concentration of 37.5 mg/mL was collected and the tracheal drip was administered to the exposed group and the Qingmai Yin intervention group. The model rats were given medicine by gavage after the dye dust. After the last exposure for 48 h, the abdominal aorta was given and the alveolar lavage fluid was collected. The activities of AKP, LDH and MDA in bronchocalveolar lavage fluid (BALF), and MDA content and SOD activity in serum were measured. Results (1) The AKP, LDH activity and MDA content in BALF of PM2.5 poisoning group increased than those in normal saline group. The activity of AKP, LDH and MDA in BALF of rats decreased after the Qingmai Yin intervention, the differences between poisoning group and Qingmai Yin intervention group were statistically significant (P<0.05); (2) After the PM2.5 exposure, the content of MDA in the serum was higher and the activity of SOD decreased than those in the normal saline group. After Qingmai Yin intervention, the content of MDA in the serum decreased and the activity of SOD decreased slightly, the difference was statistically significant (P<0.05). Conclusion PM2.5 can lead to lung inflammatory injury in rats, and Qingmai Yin has an antagonistic effect on lung inflammatory injury.