大鲵2个Sox基因HMG-box的克隆及分析

摘要

Using a pair of degenerate primers designed based on HMG-box, a conserved region in Sox gene, two 217 bp-fragments were amplified and cloned from the male and the female Andrias davidianus which exhibited no sexual difference by PCR-SSCP analysis. Amino acid sequence analysis showed one of the 217 bp-fragments showed 90% homology to Sox 11 gene of human, zebrafish, mouse, chicken, Xenopus tropicalis and Alligator sinensis respectively and the other showed 90% homology to Soxl4 gene of Tilapia, Ornithorhynchus anatinus, mouse, Fugu rubripes, zebrafish, chicken, human and Xenopus Laeuis respectively, thereafter the two fragments were termed as adSoxl 1 and adSox14c respectively. Sox gene transcripts were found in spermary, ovary, heart and kidney by RT-PCR but not stomach and liver of Andrias davidianus. These results collectively suggested that Sox gene was highly conserved across species Sox gene and was selectively expressed among tissues and organs to accommodate the need of development.%为探明Sox基因在物种进化中的保守性及其性别分型,以大鲵雌、雄个体为材料,参照Sox基因HMG-box保守区的序列,设计简并引物扩增,两因子均在雌、雄个体内得到217 bp PCR扩增产物,不存在性别差异.将二者编码的氨基酸序列与NCBI中其他物种Sox基因编码的氨基酸序列进行比对,其中一个基因与人、斑马鱼、家鼠、鸡、热带爪蟾、扬子鳄Sox11基因的同源性均为90％.另一个基因与罗非鱼、鸭嘴兽、家鼠、红鳍东方鲀、斑马鱼、鸡、人、非洲爪蟾和大鲵Sox14基因的同源性均为90％,故根据大鲵的学名,将这2个基因分别命名为adSox11和adSox14c.adSox11和adSox14c在大鲵精巢、卵巢、肾脏和心脏中均有不同程度表达,而在胃和肝脏中几乎不表达.