高通量筛选高特异性的检测类风湿关节炎的生物探针分子

摘要

从靶蛋白-配体特异性结合角度，采用蛋白折叠码（PFSC）技术在PDB数据库中高通量地筛选出与TNFA_HU⁃MAN靶点区结合灵敏度高的分子作为检测类风湿关节炎的生物探针分子.结果显示，小分子307具有相对较高的灵敏度和较低的检出限，可以作为生物探针来检测类风湿关节炎.结果显示，蛋白指纹检索技术是一种快速、有效的蛋白质结构检索工具，通过对数据库中海量数据的筛选，可为重大疾病的检测试剂开发提供先导信息.①%From the perspective protein-ligandspecific binding,the protein folding code(PFSC)will be used for high-throughput screening the molecule which is high sensitivity binding to TNFA_HUMAN target area from PDB database as biological probes molecular detection of rheumatoid disease.The results show that 307 small molecules have a relatively high sensitivity and low detection limits,so it can be used as biological probes to detect rheumatoid arthritis,also confirmed protein fingerprint retrieval technology is a rapid, efficient tool for the screening protein structure,also by screening for massive data in the database,providing information for pilot testing reagent development of major diseases.