Effects of Stromal-derived Factor 1 Preconditioning on Apoptosis of Rat Bone Mesenchymal Stem Cells

摘要

The effects of stromal-derived factor 1 preconditioning(PC)on apoptosis of bone mesenchymal stem cells(BMSCs)treated with hypoxia plus serum deprivation were investigated.Bone mesenchymal stem cells were cultured with the whole marrow-adherence technique.RT-PCR and immunohistochemistry were used to detect the expression of CXCR4.BMSCs were incubated in medium for 24 h with 10 ng/mL and 100 ng/mL SDF-1 respectively,and then they were treated with hypoxia plus serum deprivation for 6 h.Apoptosis rate was determined by flow cytometry and TUNEL method.The results showed that BMSCs had CXCR4 expression.The number of apoptotic cells was significantly reduced in SDF-1 PC group as compared with the control group,and 100 ng/mL SDF-1 PC group had the lowest level of apoptosis.It was concluded that SDF-1 preconditioning suppresses the apoptosis of BMSCs treated with hypoxia plus serum deprivation.