利用肠杆菌基因间重复序列PCR(ERIC-PCR)方法对阪崎克罗诺杆菌进行分子分型

摘要

阪崎肠杆菌在2008年被重新命名为克罗诺杆菌属的一个新种,是一种条件致病菌,可引起严重的新生儿脑膜炎、坏死性小肠结肠炎和菌血症,严重的可能引起神经系统后遗症和死亡.目前,越来越多的报道证明婴儿配方粉是其主要的传染源和传播媒介.利用肠杆菌基因间重复序列PCR (ERIC-PCR)方法对43株克罗诺杆菌属菌株和5株肠杆菌属菌株进行分子分型,利用BioNumericus软件对其结果进行分析,如果按照相似性大于80％计算,根据辛普森方程计算该分型方法的分辨率可达到0.984.研究结果表明:ERIC-PCR是一种适用于阪崎克罗诺杆菌的快速分子分型方法,能够对由该菌引起的食品中毒事件进行快速的溯源.%Cronobacter sakazakii, previously known as Enterobacter sakazakii, is Gram-negative opportunistic pathogens that can cause necrotizing enterocolitis, bacteremia and meningitis in neonates, and can possibly cause sequelae of nervous system and death in serious cases. At present, more and more reports showed that the powdered infant formulas were the main infection source and media of Cronobacter sakazakii. In this study, we used enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) to realize molecular typing of 43 Cronobacter spp and 5 Enterobacteriaceae strains, and analyzed the results by using BioNumerics version 5.0. The discriminatory index of ERIC-PCR typing method was 0.984 by Simpson's Index of Diversity basing on the similarity value of more than 80% using BioNumerics version 5.0. The results demonstrate that ERIC-PCR is a reliable, rapid typing strategy for C. sakazakii strains, and can be served as a useful tool for tracking the source of this pathogen.