纤维素酶液体混菌发酵培养基的优化研究

摘要

Using Trichoderma virde and Asperillus niger as the starting strains, the fermentation medium for complex cellulase production by mixed culture was optimized in this study. Placket-Burman experi-mental design and response surface methodology were chosen to optimize the medium. Firstly, the main factors were confirmed according to the results of PB experiments. Then, the optimum medium was obtained on this basis by response surface methodology. The optimum medium was that microcrys-talline cellulose 20 g · L-1 , corncob 20 g · L-1 , peptone 1 g · L-1 (NH4)2SO44 g · L-1 , K2HPO4 4 g · L-1 , CaCL2 ·2H2O0.5g·L-1, Tween-80 5 g · L-1. The results show that under this condi-tion, the filter paper enzyme activity and p-glucosidase activity were 2. 15 U · Ml-1 and 6. 52 U · Ml -1 respectively, which were higher than that of mono-culture and mixed culture without optimization (1.18 U · Ml-1 and 4. 99 U · Ml-1).%以绿色木霉和黑曲霉为出发菌种,采用Placket-Burman试验设计结合响应面优化的方法,对生物质水解复合纤维素酶混菌培养的培养基进行了优化.首先确定了培养基的主要因素,在此基础上通过响应面优化,确定了优化培养基组成为:微晶纤维素20 g·L-1,玉米芯20 g·L-1,蛋白胨1 g·L-1,(NH4)2SO44 g·L-1,K2HPO4 4 g·L-1,CaCl2·2H2O0.5g·L-1,Tween-80 5 g·L-1.结果表明,此条件下的滤纸酶活性和β-葡萄糖苷酶活性分别为2.15,6.52 U·mL-1,高于单菌发酵(1.18 U· mL-1)及未优化混菌发酵(4.99 U·mL-1)的最高酶活性.