内生细菌EBS05对番茄病程相关蛋白的诱导作用研究

摘要

以番茄江蔬14为材料,研究了内生细菌EBS05对番茄病程相关蛋白基因表达的诱导作用,同时,测定了番茄体内β-1,3-葡聚糖酶和几丁质酶活性变化.结果表明,内生细菌EBS05可诱导番茄体内PR1,PR2和PR3基因持续增量表达,并有效激活PR5基因表达,进而提高番茄对番茄黄化曲叶病毒(TYLCCV)的系统抗性;内生细菌EBS05诱导处理再接种TYLCCV后,番茄体内β-1,3-葡聚糖酶和几丁质酶活性均明显高于对照,并分别于接种后第7d和第9d达到活性峰值.结合TYLCCV侵染番茄的病程分析,β-1,3-葡聚糖酶和几丁质酶活性的升高与内生细菌EBS05诱导番茄对TYLCCV的系统抗性呈正相关.%Genes expression of pathogensis-related proteins (PRPs) induced by endophytic bacteria EBS05 in tomato plant were investigated using var.Jiangshu 14 as test material,and the activities of β-1,3-glueanas and chitinase were determined too.The results showed that strain EBS05 could induce the overexpression of PR1,PR2 and PR3 genes,and activate the gene PR5 to high level expression in tomato plant,thus increasing the systemic resistance to Tomato Yellow Leaf Curl China virus (TYL-CCV).The activities of β-1,3-glueanas and chitinase in tomato plants inoculated with TYLCCV after pretreated by strain EBS05 were higher than those in the control plants,and the activity peak appeared at the 9th and 7th days post inoculation,respectively.The increase of β-1,3-glueanas and chitinase activities were positively correlated with the systemic resistance to TYLCCV in tomato plants mediated by strain EBS05 based on the pathogensis of TYLCCV-infected tomato plants.