Defense Related Enzyme Induction In Coconut By Endophytic Bacteria (EPC 5)

摘要

Endophytic bacteria Bacillus subtilis (EPC 5) was isolated and tested in vitro along with Pseudomonas fluorescein (Pf 1) and the fungus Trichoderma viride (Tv 1) against Ganoderma lucidum (Leys) Karst, the causal agent of basal stem rot on coconut palm. The endophytic bacterial strains namely EPC 5 and EPC 8 showed higher vigor index (germination percentage, root and shoot length) and more inhibition against G. lucidum over un-inoculated control. These strains were confirmed as Bacillus subtilis by biochemical tests, cloning and sequencing of internal transcribed spacer (ITS) region. The Bacillus subtilis (EPC 5) along with Pseudomonas fluoresces (Pf 1) and Trichoderma viride (Tv I) has been tried as bioconsortia against basal stem rot disease undergreenhouse conditions. The soil application of bioconsortia enriched with farm yard manure (FYM) enhanced the coconut saplings growth under greenhouse conditions and showed higher induction of defense related enzymes like peroxidase, polyphenol oxidase,phenylalanine ammonia lyase and phenols when challenged with pathogen.