2种羊巴贝斯虫的核型及系统发育分析

摘要

为明确引起羊巴贝斯虫病的病原虫莫氏巴贝斯虫临潭株(Babesia motasi Lintan,BLT)及羊巴贝斯虫未定种新疆株(Babesia sp.Xinjiang,BXJ)的核型和亲缘关系,通过DNA大片段脉冲场凝胶电泳(PFGE)和三代高通量测序对2种虫体进行核型分析和系统发育分析.结果表明:2 种巴贝斯虫都有4 条染色体,但基因组的大小与核型分析不一致,莫氏巴贝斯虫临潭株基因组大小为11.1 Mb,4 条染色体大小分别为6.0 Mb、3.0 Mb、1.1 Mb和1.0 Mb;羊巴贝斯虫未定种新疆株基因组大小为7.0 Mb,4 条染色体分别为2.4 Mb、2.0 Mb、1.7 Mb和0.9 Mb;羊巴贝斯虫未定种新疆株与牛巴贝斯虫的亲缘性较近,而莫氏巴贝斯虫临潭株与双芽巴贝斯虫的亲缘关系较近.%In order to make sure karyotype and genetic relationship of Babesia motasi Lintan(BLT) and Babesia sp.Xinjiang(BXJ),the karyotype analysis and phylogenetic analysis were performed by pulsed field gel electrophoresis(PFGE) and 3 generation high-throughput sequencing.The results showed that both of them had 4 chromosomes.But the size of the genome was not consistent with karyotype analysis.Genome size of BLT was 11.1 Mb,and each chromosome size was 6.0 Mb,3.0 Mb,1.1 Mb and 1.0 Mb.Genome size of BXJ was 7.0 Mb with 4 chromosomes of 2.4 Mb,2.0 Mb,1.7 Mb and 0.9 Mb.Besides,phylogeny of the 2 parasites was analyzed on the basis of genomic data sequenced using third generation sequencing technique.Results indicated that BXJ had close genetic relationship with B.bovis while BLT was more related with B.bigemina.