169份辣椒种质资源的遗传多样性分析

摘要

基于辣椒全基因组编码区序列设计覆盖辣椒12条染色体的152对SSR引物,经过不同材料的筛选,从中挑选出条带清晰、多态性好、稳定性好的17对SSR引物,利用其对来源不同的169份辣椒材料进行遗传多样性分析,并利用POPGENE32和Phylip软件分析试验数据.结果表明:17对SSR引物共扩增出46个多态性位点,平均每对引物扩增出2.71个位点.等位基因数(Na)、有效等位基因数(Ne)、期望杂合度(He)、观测杂合度(Ho)、香农指数(I)和多态信息含量(PIC)的均值分别为2.1765、1.6291、0.3602、0.8347、0.5496、0.3106,表明辣椒遗传信息非常丰富.利用UPGMA方法对169份辣椒材料进行聚类分析,结果将材料聚为7类.%In this study 152 pairs of SSR primers were designed based on the whole genome coding region sequence of pepper. The different species of Capsicum were used to screen 152 pairs of primers,and among them 17 pairs of SSR polymorphic primers showing clear bands and good stability were obtained. The genetic diversity of different species of pepper germplasm was studied by these primers.POPGENE32 and Phylip softwares were used to analyze the experimental data.The results showed that 17 pairs of SSR primers amplified 46 polymorphic bands,with an average of 2.71 loci amplified by each pair of primers. The mean value of allele number(Na),effective allele number(Ne),expected heterozygosity(He), observed heterozygosity(Ho),Shannon-Weaver index(I) and polymorphism information content(PIC) were 2.176 5,1.629 1,0.360 2,0.834 7,0.549 6 and 0.310 6 respectively,indicating that pepper genetic information was very rich.The 169 accessions of Capsicum were analyzed using UPGMA method, and clustered into seven branches.