反硝化-厌氧氨氧化掺杂培养的厌氧氨氧化菌影响因素研究

摘要

为了得到厌氧氨氧化菌最适宜的生长环境,利用培养成熟的厌氧氨氧化颗粒污泥进行厌氧氨氧化菌的影响因素研究.探讨了温度、pH值、COD、进水基质(NH+4-N和NO-2-N)对厌氧氨氧化菌活性的影响.研究结果表明:厌氧氨氧化菌最适温度为40 ℃;最适pH值范围为7.0~8.0;COD质量浓度低于100 mg/L时,对厌氧氨氧化菌无明显抑制作用,COD质量浓度高于100 mg/L时,反硝化菌生长占据优势,一定程度上抑制了厌氧氨氧化菌的活性;进水基质NH+4-N和NO-2-N在质量浓度分别低于1 540 mg/L和140 mg/L时，厌氧氨氧化菌活性没有受到严重抑制.控制厌氧氨氧化工艺的最适生长条件,有利于厌氧氨氧化菌的快速生长,进而为厌氧氨氧化反应器的快速启动奠定基础.%In order to get the optimal growth conditions of anammox bacteria, the mature-cultured anammox granule sludge is used to investigate the influencing factors.The effects of temperature, pH value, COD and influent substrate (NO-2-N and NH+4-N) on anammox bacteria activity are investigated.The results demonstrate that the optimal temperature is 40 ℃ and the optimal pH value is between 7.0~8.0 for anammox bacteria.The anammox bacteria activity is not inhibited severely when COD concentration is lower than 100 mg/L, while the denitrifying bacteria is dominant and inhibits the anammox bacteria activity when COD concentration is higher than 100 mg/L.When the influent NH+4-N and NO-2-N concentration are lower than 1 540 mg/L and 140 mg/L respectively, the anammox bacteria activity is not inhibited severely.Controlling the optimal growth conditions of anammox technical helps the rapid growth of anammox bacteria, which establishes the foundation for the rapid start-up of anammox reactor.