右美托咪啶对脂多糖致伤大鼠肾小管上皮细胞Toll样受体4表达的影响

摘要

Objective To observe the effect of dexmedetomidine for Toll-like receptor 4 (TLR4)in renal tubular epithelial cells on rat injured by lipopolysaccharide (LPS),then to explore the possible mechanism.Methods Rat renal tubular epithelial cells were purchased, recovered,cultured,then added LPS 10μg/L and were cultured and divided into three groups, group A,the blank control group,group B added dexmedetomidine 2.5μg as control group,group C joined atipamezole 10μg and added dexmedetomidine 2.5μg after 30 min as the experimental group,all continued culture for 1 2 h.Then,immunohistochemistry was used to measure TLR4 protein expression and TLR4mRNA in three groups,and the apoptosis rate of three groups were determined.Results After using dexmedetomidine,TLR4,TLR4mRNA and apoptotic rate of group B were lower than those of A group (all P<0.01 ),while TLR4,TLR4mRNA and apoptosis rate of group C were higher than those of group B (all P<0.01 ).Conclusion Dexmedetomidine can downregulate TLR4 expression in renal tubular epithelial cells of the rat inj ured by LPS,and the regulation for TLR4 of dexmedetomidine is related withα2 adrenoglc recptor excited.%目的：观察右美托咪啶对脂多糖致伤大鼠肾小管上皮细胞Toll样受体4（Toll-like receptor 4，TLR4）表达的影响，并探讨其可能的机制。方法购买大鼠肾小管上皮细胞，经复苏、培养，均加入10μg／L脂多糖，均经培养后分为3组。A组为空白对照组；B组为阳性对照组，加入右美托咪啶2．5μg；C组作为实验组，先加入阿替美唑10μg，30 min后加入右美托咪啶2．5μg。继续培养12 h。采取免疫组织化学法检测3组细胞 TLR4表达及TLR4mRNA水平，并测定3组细胞凋亡率。结果 B组应用右美托咪啶后，TLR4、TLR4mRNA和细胞凋亡率均低于 A组（P＜0．01）；C组TLR4、TLR4mRNA 和细胞凋亡率均高于 B组（P＜0．01）。结论右美托咪啶可下调脂多糖致伤大鼠肾小管上皮细胞TLR4表达，且右美托咪啶对TLR4的调节与α2肾上腺素能受体被激动相关。