朱顶红中化学成分提取分离及其结构鉴定

摘要

选择植物朱顶红对其化学成分进行系统的提取分离和鉴定,并且研究其体外细胞毒活性,旨在能发现具有良好抗肿瘤活性化合物,为朱顶红的进一步的开发研究提供科学的依据.运用乙醇加热回流提取朱顶红干燥叶片中的化学成分.依次用石油醚、二氯甲烷、乙酸乙酯、正丁醇对朱顶红进行萃取粗分.采用MTT法检测朱顶红四部分提取物对人肝癌HepG-2细胞增殖抑制作用.对正丁醇部分浸膏利用多重色谱法进行分离纯化,得到2个单体化合物.根据所得化合物的理化性质,结合1H-NMR、13 C-NMR、MS等光谱数据鉴定出了其中的2个化合物的结构,分别为(2S)-7,3′-二羟基-4′-甲氧基黄烷((2S)-7,3′-dihydroxy-4′-methoxy-flavan),(2S)-7-羟基-3′,4′-二甲氧基黄烷((2S)-7-hydroxy-3″,4′-dimethoxy-flavan),这两个化合物为首次从朱顶红中分离得到.研究朱顶红四部分提取物对HepG-2细胞活性影响发现,朱顶红四部分提取物对人肝癌HepG-2均有显著的抑制作用,并且与剂量成正相关依赖性.%This paper aiming to provide a theoretical basis for the further development and use of hippeastrum, it is intended to study the chemical composition of hippeastrum dry leav-es and its pharmacological activity.Used heat reflux extraction to obtain chemical composi-tions in hippeastrum and then solvent extract followed by petroleum ether, methylene chlo-ride, ethyl acetate and n-butanol.Use MTT assay to detect four extracted parts on HepG-2 cells in order to choose a better inhibitive part.After separate and purify the compounds in n-butanol section by multiplex chromatography, two compounds were acquired.According to the date of 1 H-NMR, 13 C-NMR, MS and otherspectroscopic data along with the physi-cal and chemical properties, two compounds structure were identified, namely (2S) -7,3′-dihydroxy-4′-methoxy-flavan and (2S) -7 -hydroxy-3″,4′-dimethoxy-flavan. These two compounds were isolated from hippeastrum.The MTT assay results showed that the four parts extraction of amaryllis leaves significantly and dose-dependently inhabit HepG-2 cellular proliferation.