木犀草素对肝癌HepG2细胞体外增殖及血管新生活力的影响

摘要

Objective:To study the effect of luteolin on HepG2 liver cancer cell proliferation in vitro and angiogenesis activity. Methods:HepG2 liver cancer cell lines were cultured and divided into the luteolin group that were treated with serum-free medium containing 100μmol/L luteolin and the normal control group that treated with serum-free medium containing drugs. After 24 hours of treatment,MTT kit was used to detect the cell proliferation activity,and fluorescence quantitative PCR kit was used to detect the mRNA expression of pro-proliferation genes,pro-apoptosis genes and angiogenesis molecules. Results:After 24 hours of treatment,the cell proliferation activity of luteolin group was significantly lower than that of normal control group;LETM1,URG11,PICK1,CyclinD1,Uba2,VEGF,Fra-1,HIF-1α and Rac1 mRNA expression in luteolin group were significantly lower than those in normal control group while Merlin,AlkBH8,ARID1A and Panx1 mRNA expres-sion were significantly higher than those in normal control group. Conclusion:Luteolin has inhibiting effect on the HepG2 liver cancer cell proliferation in vitro and angiogenesis activity.%目的:研究木犀草素对肝癌 HepG2细胞体外增殖及血管新生活力的影响.方法:培养肝癌HepG2细胞株并分为用含有100 μmol/L木犀草素的无血清培养基处理的木犀草素组以及用含药物的无血清培养基处理的常规对照组.处理24小时后,采用M T T 试剂盒测定细胞的增殖活力值,采用荧光定量PCR试剂盒测定促增殖基因、促凋亡基因、血管新生分子的mRNA表达量.结果:处理24小时后,木犀草素组细胞的增殖活力值显著低于常规对照组;木犀草素组细胞中LETM1、URG11、PICK1、CyclinD1、Uba2、VEGF、Fra-1、HIF-1α、Rac1的mRNA表达量显著低于常规对照组,Merlin、AlkBH8、ARID1A、Panx1的mRNA表达量显著高于常规对照组.结论:木犀草素对肝癌 HepG2细胞的体外增殖活力以及血管新生活力具有抑制作用.