The F protein of Hepatitis C virus subtype 1b inhibits human hepatocellular carcinom HepG2 cell apoptosis in vitro

摘要

To investigate the effects of the F protein of Hepatitis C virus subtype lb on the apoptosis of human hepatocellular carcinom HepG2 cells. HepG2 cells were transfected with recombinant plasmid pcDNA3.0-F-EGFP. And pcDNA3.0-F-EGFP-HepG2 strain was exposed to Act-D and tumor necrosis factor α (TNFα) treatment to induce cell apoptosis with positive control pcDNA3.0-C-EGFP-HepG2, negative control pcDNA3.0-C-EGFP-HepG2and blank control HepG2. Annexin V-FITC/PI of Flow cytometry was performed to determine the number of apoptotic cells. DNA Ladder was used to observe the isolation of apoptotic DNA fragments in the apoptotic cells. pcDNA3.0-F-EGFP-HepG2cell strain showed a much delayed apoptosis as well as obviously lowered apoptotic rate in comparison with the pcDNA3.0-HepG2 strain and HepG2 strain (P<0.01). The introduction and expression of extraneous gene (the F gene of Hepatitis C virus subtype 1b) significantly inhibits the apoptosis of HepG2 cells.