Effect of luteolin on HepG2 liver cancer cell proliferation in vitro and angiogenesis activity

摘要

Objective: To study the effect of luteolin on HepG2 liver cancer cell proliferation in vitro andangiogenesis activity. Methods: HepG2 liver cancer cell lines were cultured and divided intothe luteolin group that were treated with serum-free medium containing 100 μmol/L luteolinand the normal control group that treated with serum-free medium containing drugs. After24 h of treatment, MTT kit was used to detect the cell proliferation activity, and fluorescencequantitative PCR kit was used to detect the mRNA expression of pro-proliferation genes,pro-apoptosis genes and angiogenesis molecules. Results: After 24 h of treatment, the cellproliferation activity of luteolin group was significantly lower than that of normal controlgroup; LETM1, URG11, PICK1, CyclinD1, Uba2, VEGF, Fra-1, HIF-1α and Rac1 mRNAexpression in luteolin group were significantly lower than those in normal control group whileMerlin, AlkBH8, ARID1A and Panx1 mRNA expression were significantly higher than thosein normal control group. Conclusion: Luteolin has inhibiting effect on the HepG2 liver cancercell proliferation in vitro and angiogenesis activity.