鸦胆子油注射液联合新辅助化疗对胃癌患者恶性分子表达及抗肿瘤免疫应答的影响

摘要

目的:研究鸦胆子油注射液联合新辅助化疗对胃癌患者恶性分子表达及抗肿瘤免疫应答的影响.方法:选择在我院接受术前新辅助化疗的78例胃癌患者并随机分为两组,干预组接受鸦胆子油注射液联合新辅助化疗,对照组接受新辅助化疗.治疗前后测定血清肿瘤标志物的含量、外周血调节分子的表达量,手术后测定胃癌病灶内恶性分子的表达量.结果:治疗后2个周期、4个周期时,两组患者血清中CEA、DKK1、exosc2、ANXA2的含量均显著低于治疗前,对照组患者外周血单个核细胞中PD-1、TIM-3、Foxp3的mRNA表达量显著高于治疗前,干预组患者外周血单个核细胞中PD-1、TIM-3、Foxp3的mRNA表达量显著低于治疗前且干预组患者血清中CEA、DKK1、exosc2、ANXA2的含量以及外周血单个核细胞中PD-1、TIM-3、Foxp3的mRNA表达量均显著低于对照组,胃癌病灶内GKN1、GKN2的mRNA表达量显著高于对照组,GOLPH3、PTP1B的mRNA表达量显著低于对照组.结论:鸦胆子油注射液联合新辅助化疗能够更为有效地杀伤胃癌细胞、改善抗肿瘤免疫应答.%Objective: To study the effect of brucea javanica oil injection combined with neoadjuvant chemotherapy on malignant molecule expression and antitumor immune response in patients with gastric cancer.Methods: 78 patients with gastric cancer undergoing preoperative neoadjuvant chemotherapy in our hospital between May 2013 and July 2016 were selected and randomly divided into two groups, intervention group received brucea javanica oil injection combined with neoadjuvant chemotherapy, and the control group accepted neoadjuvant chemotherapy.Serum tumor marker levels and peripheral blood regulatory molecule expression were determined before and after treatment, and the malignant molecule expression levels in gastric cancer lesions were determined after the operation.Results: Two cycles and 4 cycles after treatment, serum CEA, DKK1, exosc2 and ANXA2 levels of both groups of patients were significantly lower than before treatment, PD-1, TIM-3 and Foxp3 mRNA expression in peripheral blood mononuclear cells of control group were significantly higher than those before treatment, PD-1, TIM-3 and Foxp3 mRNA expression in peripheral blood mononuclear cells of intervention group were significantly lower than those before treatment, serum CEA, DKK1, exosc2 and ANXA2 levels as well as PD-1, TIM-3 and Foxp3 mRNA expression in peripheral blood mononuclear cells of intervention group were significantly lower than those of control group, and the GKN1 and GKN2 mRNA expression in gastric cancer lesions were significantly higher than those of control group while GOLPH3 and PTP1B mRNA expression were significantly lower than those of control group.Conclusion: Brucea javanica oil injection combined with neoadjuvant chemotherapy can more effectively kill the gastric cancer cells and improve the antitumor immune response.