芍药苷通过TLR4炎症通路和Nrf2氧化应激通路减轻大鼠脊髓损伤的实验研究

摘要

Objective: To study the effecof paeoniflorin on TLR4 inflammatory pathway and Nrf2 oxidative strespathway in ramodel with spinal cord injury.Methods: Male SD ratwere selected and divided into sham group, spinal cord injury group and paeoniflorin group, spinal cord injury ramodelwere established and then given 30 mg/kg paeoniflorin fo14 d of intervention in row, and then serum and spinal cord specimenwere collected to determine the expression of cell apoptosigenes, TLR4 pathway geneand Nrf-2 pathway genes.Results: Spinal cord caspase-3, caspase-8, caspase-9, TLR4, MyD88, NF-kB, AP-1, Nrf-2 and ARE mRNexpression awell aserum IL-6, TNF-α and IL-12 contentof spinal cord injury group were significantly highethan those of sham group while spinal cord HO-1, SOD and GSH-PX contentwere significantly lowethan those of sham group;spinal cord caspase-3, caspase-8, caspase-9, TLR4, MyD88, NF-kand AP-1 mRNexpression awell aserum IL-6, TNF-α and IL-12 contentof paeoniflorin group were significantly lowethan those of spinal cord injury group while spinal cord Nrf-2 and ARE mRNexpression awell aHO-1, SOD and GSH-PX contentwere significantly highethan those of spinal cord injury group.Conclusions: Paeoniflorin can inhibithe TLR4 inflammatory pathway and enhance the Nrf2 anti-oxidative strespathway to reduce the spinal cord injury and inhibicell apoptosiin rats.%目的:研究芍药苷对脊髓损伤大鼠模型TLR4炎症通路和Nrf2氧化应激通路的影响.方法:选择雄性SD大鼠并分为假手术组、脊髓损伤组、芍药苷组,建立脊髓损伤大鼠模型后给予30 mg/kg芍药苷连续干预14天,而后取血清标本和脊髓标本,测定细胞凋亡基因、TLR4通路基因、Nrf-2通路基因的表达量.结果:脊髓损伤组大鼠脊髓中caspase-3、caspase-8、caspase-9、TLR4、MyD88、NF-kB、AP-1、Nrf-2、ARE的mRNA表达量以及血清中IL-6、TNF-α、IL-12的含量显著高于假手术组,脊髓中HO-1、SOD、GSH-PX的含量显著低于假手术组;芍药苷组大鼠脊髓中caspase-3、caspase-8、caspase-9、TLR4、MyD88、NF-kB、AP-1的mRNA表达量以及血清中IL-6、TNF-α、IL-12的含量显著低于脊髓损伤组,脊髓中Nrf-2、ARE的mRNA表达量以及HO-1、SOD、GSH-PX的含量显著高于脊髓损伤组.结论:芍药苷能够通过抑制TLR4炎症通路、增强Nrf2抗氧化应激通路来减轻大鼠脊髓损伤、抑制细胞凋亡.