AP-4、EZH2基因表达量与子宫内膜癌病灶中细胞凋亡、上皮间质转化的相关性研究

摘要

Objective:To study the correlation of AP-4 and EZH2 gene expression with apoptosis and epithelial-mesen-chymal transition in endometrial cancer lesions .Methods:Patients with endometrial cancer who received surgical resection in Xiaogan First People's Hospital between February 2015 and January 2017 were selected ,right amount of endometrial cancer lesion and adjacent lesion was collected to extract RNA ,and then the expression of AP-4 and EZH2 gene as well as apoptosis genes and epithelial-mesenchymal transition genes were determined .Results:AP-4 and EZH2 gene mRNA expression in endo-thelial cancer lesion were greatly higher than those in adjacent lesion ;SRPX2 ,RLIP76 ,ZEB1 ,SALL4 ,TET1 ,RANKL and N-cadherin mRNA expression in endometrial cancer lesion were greatly higher than those in adjacent lesions whereas Fat-1 , ITLN-1 ,Caspase-3 and α-catenin mRNA expression were greatly lower than those in adjacent lesions ;SRPX2 and RLIP76 mRNA expression in endometrial cancer lesion with high AP-4 expression were greatly higher than those in endometrial cancer lesion with low AP-4 expression whereas Fat-1 ,ITLN-1 and Caspase-3 mRNA expression were greatly lower than those in en-dometrial cancer lesion with low AP-4 expression;ZEB1 ,SALL4 ,TET1 ,RANKL and N-cadherin mRNA expression in endo-metrial cancer lesion with high EZH2 expression were greatly higher than those in endometrial cancer lesion with low EZH2 ex-pression whereas α-catenin mRNA expression was greatly lower than that in endometrial cancer lesion with low EZH 2 expres-sion .Conclusion:The AP-4 and EZH2 gene highly expressed in endometrial cancer lesion can inhibit the apoptosis of cancer cells and promote the epithelial-mesenchymal transition of cancer cells .%目的:研究A P-4、EZ H2基因表达量与子宫内膜癌病灶中细胞凋亡、上皮间质转化的相关性.方法:选择在孝感市第一人民医院接受手术切除治疗的子宫内膜癌患者,收集子宫内膜癌病灶及癌旁病灶适量,抽提RNA后测定AP-4、EZH2基因及凋亡基因、上皮间质转化基因的表达量.结果:子宫内膜癌病灶内AP-4、EZH2基因的mRNA表达量显著高于癌旁病灶;子宫内膜癌病灶内SRPX2、RLIP76、ZEB1、SALL4、TET1、RANKL、N-cadherin的mRNA表达量显著高于癌旁病灶,Fat-1、ITLN-1、Caspase-3、α-catenin的mRNA表达量显著低于癌旁病灶;AP-4高表达的子宫内膜癌病灶内SRPX2、RLIP76的mRNA表达量显著高于AP-4低表达的子宫内膜癌病灶,Fat-1、ITLN-1、Caspase-3的mR-NA表达量显著低于AP-4低表达的子宫内膜癌病灶;EZH2高表达的子宫内膜癌病灶内ZEB1、SALL4、TET1、RANKL、N-cadherin的mRNA表达量显著高于EZH2低表达的子宫内膜癌病灶,α-catenin的m RN A表达量显著低于EZ H2低表达的子宫内膜癌病灶.结论:子宫内膜癌病灶内高表达的A P-4、EZ H2基因分别能够抑制癌细胞凋亡、促进癌细胞上皮间质转化.