急性肺损伤小鼠肺泡巨噬细胞TREM1 mRNA的表达及意义

摘要

目的: 研究脂多糖(LPS)致急性肺损伤(ALI)小鼠肺泡巨噬细胞(AM)髓系细胞触发受体1 (TREM1)mRNA的表达及意义.方法:48只小鼠分成对照组和实验组,对照组腹腔注射生理盐水,实验组腹腔注射LPS诱导ALI模型;于灌注后3、6、12及24 h,采用荧光定量逆转录(RT-PCR)方法检测支气管肺泡灌洗液中提取的AM中TREM1 mRNA表达水平,用ELISA法检测支气管肺泡灌洗液上清液中的TNFα、可溶性TREM1(sTREM1)水平,并分析TREM1 mRNA与sTREM1和TNFα表达水平的相关性.结果:各对照组AM均表达TREM1 mRNA,但各时间点比较差异无统计学意义(P>0.05);观察组TREM1 mRNA、TNFα及sTREM1的表达均在3 h时上升致最高,之后下降;TREM1mRNA与sTREM1及TNFα的表达水平呈正相关(r=0.783,P<0.001;r=0.472,P<0.05).结论:ALI小鼠支气管肺泡灌洗液、及其AM中TREM1 mRNA表达水平升高,TREM1 mRNA升高与sTREM1、TNFα呈正相关.%Objective: To investigate the expression and clinical significance of TREM1 mRNA in the alveolar macrophages (AM) of acute lung injury induced by lipopolysaccharide (LPS) in rat.Methods:48 rats were randomly divided into control group and experiment group, control group was intraperitoneally injected with normal saline;experiment group was intraperitoneally injected with LPS to induce ALI model.After 3, 6, 12 and 24 h, RT-PCR was adopted to detect TREM1 mRNA expression level in AM extracted from bronchoalveolar lavage fluid.ELISA was used to detect the concentration of TNF-α and soluble TREM1 level in supernatant of bronchoalveolar lavage fluid;analyzing correlation between expression level of TREM1 mRNA and sTREM1 together with TNFα.Results:AM of control group expressed TREM1 mRNA, while comparison differences of each time point showed no statistical significance(P>0.05);the expression of TREM1 mRNA, TNFα and sTREM1 rose to the highest in 3h, then declined;expression of TREM1 mRNA and TNFα and sTREM1 level were positively correlated(r=0.783,P<0.001 and r=0.472,P<0.05).Conclusion:ALI rat bronchoalveolar lavage fluid and expression level of TREM1 mRNA increased, the increase of TREM1 mRNA was positively correlated with sTREM1 and TNFα.