IMMORTALIZED PORCINE ALVEOLAR MACROPHAGE CELL LINE AND A METHOD FOR DETECTING AND PROLIFERATING THE PORCINE ALVEOLAR MACROPHAGE INFECTIOUS VIRUS

摘要

PURPOSE: An immortalized porcine alveolar macrophage and a method for detecting and proliferating infectious virus using the same are provided to develop a diagnostic method and a vaccine.;CONSTITUTION: An immortalized porcine alveolar macrophage is prepared by transfection of an expression vector containing hTERT(human telomerase reverse transcriptase) gene of sequence number 1 to porcine alveolar macrophage cell line. The immortalized porcine alveolar macrophage cell line is used for detecting porcine alveolar macrophage infectious virus. The virus includes porcine reproductive respiratory syndrome virus, porcine circovirus, animal and human influenza virus, porcine cytomegalo virus, vesicular stomatitis virus, aujeszky virus, swine fever virus, or vaccinia virus.;COPYRIGHT KIPO 2012