板蓝根抗甲1型流感病毒活性物质的初筛研究

摘要

[目的]观察不同提取工艺及方法制备的板蓝根样品的抗流感病毒作用,以筛选板蓝根中抗流感病毒的有效成分及活性物质.[方法]于狗肾细胞(MDCK)上接种人甲1型流感病毒PR8株(A/PR/8/34,H1N1),采用细胞病变抑制法(CPE法),在不同试验策略下(保护、治疗模式)评价不同提取工艺及方法制备的板蓝根样品的体外抗流感病毒作用.[结果]在无毒浓度下,从不同工艺制备的板蓝根样品中筛选到1份有抗流感病毒活性的成分,该成分为醇溶液通过大孔树脂用纯水洗脱的洗脱液(S-03),其体外抗病毒选择指数为 4.8.[结论]发现板蓝根样品中抗流感病毒作用较为明确的成分.%Objective The anti-influenza activity of Radix Isatidis extracts processed by different extractions was observed for the screening of anti-influenza active constituents. Methods Madin - Darby canine kidney cell line was inoculated with influenza virus type 1 PR8 strain (A/PR/8/34, H1N1). The anti-influenza activity of Radix Isatidis extracts processed by different extractions was evaluated according to cytopathic effect (CPE). Results At non-toxic concentration, one antiviral active constituent (S-03) was obtained from different extractives of Radix Isatidis. S-03 was the eluant eluted with pure water through the macroporous resin column, and its anti-viral selective index (SI) was 4.8 in vitro. Conclusion One antiviral constituent has been found in Radix Isatidis extracts.