宣肺中药对慢传输型便秘小鼠肺肠组织神经肽的影响

摘要

Objective To observe the effect of lung-dispersing herbs on pulmonary and intestinal neurokinin A (NKA), vasoactive intestinal peptide (VIP) and trefoil factor 3 (TFF3) contents in mice with slow transit constipation, and to explore the connective mechanism of the lung and the large intestine internally and externally related to each other at neuropeptide level. Methods Forty mice were randomized into normal group, model group, medication model group and medication normal group. Slow transit constipation was induced with gastric gavage of self-feces and Compound Diphenoxylate in mice. Modified Jigeng Decoction was given orally for the mice in the two medication groups, and distilled water was given orally for the mice in the normal group and the model group for 7 days. On the eighth day, the mice were executed, and the pulmonary and intestinal NKA, VIP and TFF3 contents were examined. Results Compared to the normal group, the pulmonary and intestinal NAK content was obviously increased (P <0. 01) , and VIP content was obviously decreased (P <0. 05 or P <0. 01) in the model group. The pulmonary and intestinal NAK content was obviously decreased (P < 0. 01 ), and pulmonary VIP content was obviously increased (P < 0. 05 or P < 0. 01 ) in the medication model group as compared with those in the model group. The pulmonary and intestinal TFF3 content showed no obvious changes in all of the groups. The results of Spearman correlation analysis showed that the pulmonary and intestinal NKA, VIP and TFF3 contents were positive correlated, and the correlation coefficient was 0.448, 0.503, 0.423, respectively. Conclusion Modified Jigeng Decoction, which has the action of dispersing lung, shows certain effect on improving the pathological state of mice with slow transit constipation, and its therapeutic mechanism is probably related with the regulation of the pulmonary and intestinal NKA and VIP contents.%[目的]观察宣肺中药对慢传输型便秘小鼠肺、肠组织神经激肽A(NKA)、血管活性肠肽(VIP)、肠三叶因子( TFF3)含量的影响,从神经肽角度探讨“肺合大肠”脏腑相关联络机制.[方法]将40只小鼠随机分为正常组、模型组、模型给药组及正常给药组,采用自身粪便与复方地芬诺酯(剂量为2 mg/kg)联合灌胃法复制慢传输型便秘模型.模型给药组及正常给药组给予加味桔梗汤灌胃(剂量为12g·kg-1·d-1),正常组、模型组则以蒸馏水灌胃,连续7d.第8天处死动物,测定肺、肠组织中NKA、VIP和TFF3含量.[结果]与正常组比较,模型组小鼠肺、肠组织中NKA含量均显著升高(P＜0.01),VIP含量均显著降低(P＜0.05或P＜0.01)；与模型组比较,模型给药组肺、肠组织中NKA含量均显著降低,肺组织中VIP含量显著升高(P＜0.05或P＜0.01)；各组小鼠肺、肠组织TFF3含量均无显著变化.Spearman简单相关分析结果显示:肺、肠组织中NKA、VIP、TFF3含量均呈显著相关关系,其相关系数分别为0.448、0.503、0.423.[结论]宣肺中药加味桔梗汤可改善慢传输型便秘的病理状态,其效应机制可能与调节肺、肠组织中神经肽NKA、VIP的含量密切相关.