血清c-Myc与EB病毒抗体联合检测对鼻咽癌的诊断价值

摘要

目的:探讨原癌基因c-Myc与EB病毒(EBV)抗体联合检测对鼻咽癌(NPC)的临床诊断价值.方法:采用酶联免疫吸附试验(ELISA)法对68例NPC患者(观察组)及20例健康体检者(对照组)血清中的c-Myc、EBV衣壳抗原IgA抗体(EB-VCA-IgA)和EBV早期抗原IgA抗体(EBV-EA-IgA)水平进行检测,观察血清c-Myc、EBV抗体浓度与患者临床病理特征的关系,并采用受试者工作特征(ROC)曲线评估c-Myc、EBV-VCA-IgA和EBV-EA-IgA对NPC的诊断价值.结果:观察组患者血清c-Myc、EBV-VCA-IgA和EBV-EA-IgA浓度均明显高于对照组(均P＜0.05).晚期(Ⅲ～Ⅳ期)NPC患者血清中EBV-VCA-IgA的浓度较早期(Ⅰ～Ⅱ期)高,T3～T4期较T1～T2期高(P＜0.05);N1～N3期患者血清EBV-EA-IgA水平明显高于N0期(P＜0.05).单项检测c-Myc、EBV-VCA-IgA和EBV-EA-IgA的ROC曲线下面积(AUC)分别为0.671、0.844、0.697,三者联合检测的AUC为0.879.结论:c-Myc、EBv-VCA-IgA和EBV-EA-IgA联合检测对NPC的诊断具有较高的临床价值.%Objective:To investigate the clinical value of combined detection of c-Myc and Epstein-Barr virus (EBV) antibodies in nasopharyngeal carcinoma (NPC).Methods:The serum levels of c-Myc,EBV-VCA-IgA and EBV-EA-IgA were detected by ELISA in 68 patients with NPC and 20 healthy subjects.Results:The serum levels of c-Myc,EBV-VCA-IgA and EBV-EA-IgA in NPC patients were higher than those of controls (P＜ 0.05).EBV-VCA-IgA was related to clinical stage and T stage (P＜ 0.05),EBV-EA-IgA was associated with N stage (P＜ 0.05).The areas under the ROC curve (AUC)of c-Myc,EBV-VCA-IgA and EBV-EA-IgA were 0.671,0.844 and 0.697,respectively.The AUC of the combination detection of c-Myc,EBV-VCA-IgA and EBV-EA-Ig was 0.879.Conclusion:Combined detection of c-Myc,EBV-VCA-IgA and EBV-EA-IgA was valuable in the diagnosis of NPC.