急性移植物抗宿主病患者血浆miR-155的表达及其临床意义

摘要

Objective:To evaluate the expression and clinical significance of miR-155 in patients with acute graft-versus-host disease (aGVHD).Methods:miRNA was extracted from the plasma samples of patients day 0,day 7,day 14,day 21,day 28 after their hematopoietic stem cell transplantation(HSCT).The expressions of miR-155,interleukin (IL)-4,IL-7 and IL-l 0 were detected by qPCR.Results:The expression of miR-155 obviously increased on day 7 after HSCT,and reached the peak on day 28.The miR-155 expression level was increased with the prolonged time after transplantation (P＜ 0.05).There was no significant difference in the expression of IL-4,IL-7 and IL-10 between any time points (P＞ 0.05).Conclusion:MiR-155 might be a noninvasion biomarker for early detection of aGVHD,and the level of miR-155 changed earlier than 1L-4,IL-10 and IL-7 after HSCT.%目的:探讨急性移植物抗宿主病(aGVHD)患者miR-155的表达水平及其临床意义.方法:采用造血干细胞移植患者移植第0、第7、第14、第21及第28天的血浆标本,实时荧光定量PCR (qPCR)方法检测miR-155相对表达量,酶联免疫吸附试验(ELISA)法检测患者血浆中白细胞介素(IL)-4、IL-7、IL-10的含量.结果:miR-155在移植后第7天开始逐渐升高,到第28天时升高明显,患者移植后不同时间点miR-155相对表达量比较差异具有统计学意义(P＜0.05).allo-HSCT后不同时间点IL-4、IL-7、IL-10水平比较,差异均无统计学意义(P＞0.05).结论:allo-HSCT后miR-155较IL-4、IL-7、IL-10水平变化出现早,可作为早期诊断和预测aGVHD的无创性生物标记物.