IGF-Ⅰ和IGF-Ⅱ在子宫肌瘤患者血清中的水平及其与肿瘤组织ER、PR表达的相关性分析

摘要

Objective:To explore the correlation of serum levels of insulin-like growth factor (IGF)-Ⅰ and IGF-Ⅱ and the expressions of estrogen receptor (ER) and progestrone receptor (PR) in tumor tissues of patients with uterine leiomyoma.Methods:84 patients with uterine leiomyoma treated in our hospital from September 2014 to August 2015 were selected in the study group,and 84 healthy women who had health examination in our hospital were enrolled in the control group.The serum levels of IGF-Ⅰ and IGF-Ⅱ was measured by enzyme linked immunosorbent assay (ELISA).The expression levels of EP and PR in tumor tissues were detected by immunohistochemistry.Results:The levels of IGF-Ⅰ and IGF-Ⅱ in the study group were higher than those in the control group (P＜0.01).Patients with positive ER and PR had higher levels of IGF-Ⅰ and IGF-Ⅱ than those with negative ER and PR (P＜0.01).Pearson correlation analysis showed that the serum levels of IGF-Ⅰ and IGF-lⅡ were positively correlated with the expressions of ER and PR in tumor tissues (P＜0.01).Conclusion:The levels of IGF-Ⅰ and IGF-Ⅱ in serum were markedly increased in patients with uterine leiomyoma,and were positively associated with the expressions of ER and PR in tumor tissues.Therefore,they might influence the occurrence and development of uterine leiomyoma.%目的:探讨胰岛素样生长因子(IGF)-Ⅰ和IGF-Ⅱ在子宫肌瘤患者血清中的水平及其与肿瘤组织雌激素受体(ER)、孕激素受体(PR)表达的相关性.方法:选取2014年9月至2015年8月青岛大学附属青岛妇女儿童医院收治的84例子宫肌瘤患者为研究组,另选取同期在本院行健康体检的84例健康妇女为对照组.采用酶联免疫吸附试验(ELISA)法检测两组外周血IGF-Ⅰ和IGF-Ⅱ水平,免疫组化法检测研究组肿瘤组织ER、PR的表达,分析IGF-Ⅰ和IGF-Ⅱ与患者肿瘤组织中的ER、PR表达的相关性.结果:研究组血清IGF-Ⅰ和IGF-Ⅱ水平均明显高于对照组(均P＜0.01);ER(+)、PR(+)患者血清中IGF-Ⅰ和IGF-Ⅱ水平均显著高于ER(-)、PR(-)患者(均P＜0.01);Pearson相关性分析结果显示,子宫肌瘤患者血清IGF-Ⅰ和IGF-Ⅱ水平与子宫肌瘤组织中ER、PR表达均呈正相关关系(均P＜0.01).结论:子宫肌瘤患者血清中的IGF-Ⅰ和IGFⅡ水平升高,两者与肿瘤组织ER、PR的表达呈正相关关系,并可能影响子宫肌瘤的发生和发展.