TRANSFECTED CELL LINE FOR EXPRESSION OF IGF- PROMOTER AND MEATHOD TO MEASURE THE ACTIVITY OF IGF- PROMOTER SITE USING THEREIT

摘要

In the present invention, HEK (Human Embryonic Kidney) in 293 cells, IGF-Ⅰ and pIGF plasmid having a promoter region, in combination with pCINeo vector having a gene showing resistance to neomycin (neomycin) by carrying out the trans faction (transfection) disclosed, and further, by using the cells, measuring the IGF-ⅰ promoter region of the activity in accordance with the fluorescence method of transfected cell lines PRDCIGF 1 (KCLRF-BP-00091) expressing IGF-ⅰ promoter, characterized in that the obtained It discloses a method for. Transfected cell lines expressing IGF-Ⅰ promoter according to the present invention may pIGF is stably expressed, easily search a substance which induces the transcription of IGF-Ⅰ. And, IGF-Ⅰ activity measuring method of the promoter region according to the present invention, unlike the method of measuring the conventional IGF-Ⅰ, and a substance which induces the transcription of IGF-Ⅰ at the cellular level can be easily search, it takes its measurements the cost is also cheaper. Thus, the transfected cell lines and IGF-Ⅰ activity measuring method of the promoter region to express the IGF-Ⅰ promoter according to the present invention may be useful for searching a substance which promotes the formation of IGF-Ⅰ and study its effect.