经皮神经电刺激对大鼠切口痛的镇痛效果及血浆炎症因子的影响

摘要

Objective:To observe the effects of transcutaneous electrical nerve stimulation (TENS) on incision pain and plasma inflammatory factors levels in rats.Methods:66 Wistar rats were randomly divided into 3 groups:a control group,a model group and a TENS group.The rats were subjected to incision pain except for the controls.At 4 h after modeling,the rats in the TENS group were treated with TENS.The threshold of pain was detected by tail-flick test.The levels of tumor necrosis factor-α (TNF-α),interleukin (IL)-1 and IL-6 in plasma were measured by enzyme linked immunosorbent assay (ELISA).Results:After TENS treatment,the pain threshold in TENS group was significantly increased (P ＜0.05).The plasma levels of TNF-α,IL-1 and IL-6 were notably increased in the model group compared with the control group (P ＜0.05),but were decreased in the TENS group (P ＜0.05).Conclusion:TENS had a significant an algesic effect on incision pain in rats,possibly by inhibiting the release of inflammatory factors like TNF-α,IL-1 and IL-6.%目的:探讨经皮神经电刺激(TENS)对大鼠切口痛的镇痛作用及其机制.方法:将66只Wistar大鼠随机分为3组:空白对照组、模型组、TENS组.模型组和TENS组大鼠复制切口疼痛模型.TENS组大鼠于造模后4h开始给予TENS治疗,采用辐射热甩尾法,检测大鼠逃尾反应的潜伏时间(痛阈值).用酶联免疫吸附试验(ELISA)法检测术后4h、1d、3d、7d、10d大鼠血浆中肿瘤坏死因子α(TNFα)、白介素(IL)-1、IL-6的含量.结果:与治疗前比较,TENS组治疗后大鼠痛阈值显著增高(P ＜0.05).模型组大鼠血浆中TNF-α、IL-1、IL-6水平显著高于空白对照组(P＜0.05);与模型组比较,TENS组大鼠血浆中TNF-α、IL-1、IL-6水平显著降低(P＜0.05).结论:TENS对大鼠切口痛的镇痛效果显著,该作用可能与减少促炎因子TNF α、IL-1、IL 6等的产生有关.