一株具有降解性能的巨大芽孢杆菌的鉴定与发酵条件优化

摘要

Objective]A bacterium named B1 1 capable of organic-pollutants degradation,iso-lated from sea cucumber external culturing ponds,was identified and the liquid fermentation condition of B1 1 was optimized.[Methods]The genus of B1 1 was studied through physiologi-cal and bio-chemical tests and 16S rDNA sequence similarity analysis.The condition of liquid fermentation for bacterium B1 1 were optimized by mono-factor experiment and uniform de-sign.[Results]B1 1 was preliminarily identified Bacillus megaterium.The optimized fermen-tation condition was at pH 8 with 70 mL production medium inoculated with 5% seed culture and incubated at 28℃,180 r/min.[Conclusion]B11 grew well under optimal fermentation conditions and it could satisfy the requirement for industrial large-scale cultivation,which shows a good prospect for industrial application in future.%【目的】对从刺参养殖池塘中分离得到的降解菌株 B11进行菌种鉴定,并对其发酵条件进行优化。【方法】经生化及16 S rDNA鉴定该菌种的种属；利用单因素筛选和正交试验设计对其发酵条件进行研究。【结果】经鉴定该菌株为一株巨大芽孢杆菌,最优发酵条件是装液量为70 mL/250 mL,转速为180 r/min,培养温度为28℃,pH 值为8。【结论】菌株B11在最优发酵条件下生长良好,满足工业化大规模扩大培养所需条件,有较好的应用前景。