苯胺红标记分光光度法测定微量血清白蛋白

摘要

A spectrophotometric method was proposed for the determination of Human Serum Albumin （HSA）. The method was based on the effect of amount of BSA or HSA on the increased absorbance of Aniline Red （AR） in B-R buffer at pH 4.50. The linear ranges were 0- 15.0/μg/mL and 0-12.0μg/mL for BSA and HSA, respectively. The relative coefficients was 0. 9978 and 0. 9949 for BSA and HSA, respectively. The method is simple, fast, and of high selectivity. The proposed method was applied to the determination of the total protein in human serum samples with satisfactory results.%在pH4．5的B-R缓冲溶液中，有机染料苯胺红与牛血清白蛋白（BSA）、人血清白蛋白（HSA）的结合反应进行了初步研究．结果表明：苯胺红在540nm处有一强的吸收峰，当加入BSA和HSA作用后该吸收峰的强度升高，峰位基本不变，且没有新的吸收峰出现．在此波长下测定其复合物的吸光度，其吸光度的增加值（△A）与BSA和HSA分别在0～15．0μg／mL（r=0．9978）和0～12．0μg／mL（r=0．9949）呈良好的线性关系．该方法用于实际试样测定，结果令人满意．