研究有机染料茜素红S（alizarin red S，ARS）与牛血清白蛋白（bovine serum albumin，BSA）、人血清白蛋白（human serum albumin，HSA）的结合反应，选择实验的最佳条件：PH=5．10的B—R缓冲溶液3．00mL，4．00mL5．0×10-4mol／L茜素红S溶液．反应20min后，体系的吸光度很稳定，在入λ=420nm处有最大吸收峰，并且随着牛血清白蛋白（BSA），人血清白蛋白（HSA）的加入，茜素红S的吸收峰下降．因此以茜素红S为标记物，根据其在波长420nm处吸收峰下降的程度，可用于定量测定BSA和HSA．测量BSA的线性响应范围为0～32．0μg／mL，相关系数为0．9987，测量BSA的线性响应范围为0～28．0μg／mL，相关系数为0．9968．该方法具有较高的灵敏度和选择性，用于实际试样分析，实验结果令人满意．%The protein is a kind of important biological macromolecules in the human body. It is not merely very important and essential for maintaining the life. A acidic dye has been applied to the assay of proteins in the fields of medicine and life science. The reactions of human serum albumen （HSA） and bovine serum albumen （BSA） with alizarin red have been studied. Both BSA and HSA can form orange complexes, which decrease the absorbance. The optimum conditions of complex reactions of a new chromogenic reagent alizarin red with BSA and HSA was studied. The method was used in the photometric determination of BSA and HSA. In a buffer medium of pH 5.10, alizarin red reacts with BSA and HSA to form a complex having its absorption maxima at 420nm and the reactions steady with 80 rain. Beefs law is obeyed in the concentration in the range of 0-32.0 μg/mL（r=0. 998 7） for BSA and 0-28.0μg/mL（r= 0. 996 8） for HSA. The BSA and HSA in human serum samples were determined, and the result is satisfactory.
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