首页> 外国专利> MICROSATELLITE MARKER FOR IDENTIFYING SPECIES OF RED MOLD PATHOGEN AND METHOD FOR IDENTIFYING SPECIES OF RED MOLD PATHOGEN USING THE MARKER

MICROSATELLITE MARKER FOR IDENTIFYING SPECIES OF RED MOLD PATHOGEN AND METHOD FOR IDENTIFYING SPECIES OF RED MOLD PATHOGEN USING THE MARKER

机译:用于鉴别红霉病原菌种的微卫星标记物和使用该标记物鉴别红霉病菌种的方法

摘要

PURPOSE: A microsatellite marker for distinguishing species according to the type of toxin generated from red mold pathogen and a method for distinguishing species of pathogen using the same are provided to quickly and accurately distinguish main species and toxin by analyzing PCR using FgmsGT28. CONSTITUTION: A primer, FgmsGT28, of sequence numbers 1 and 2 is able to specifically amplify a microsatellite of red mold pathogen to distinguish Fusarium graminearum which produces deoxynivalenol(DON) and Fusarium asiaticum which produces nivalenol(NIV) by PCR(Polymerization Chain Reaction). A method for distinguishing F. graminearum and F. asiaticum comprises: a step of amplifying a gene using the primer by PCR; and a step of identifying a microsatellite site of the amplified gene. A kit for diagnosing and distinguishing species of red mold pathogen contains the primer and a reagent for amplification.
机译:目的:提供一种根据红霉菌病原体产生的毒素类型区分物种的微卫星标记,以及使用该标记物区分病原体种类的方法,以通过使用FgmsGT28分析PCR来快速,准确地区分主要种类和毒素。组成:序列号为1和2的FgmsGT28引物能够特异性扩增红色霉菌病原体的微卫星,以区分产生脱氧雪茄烯醇(DON)的禾谷镰刀菌和通过PCR(聚合链反应)产生雪茄烯醇(NIV)的亚洲镰刀菌。 。一种用于鉴别禾本科镰刀菌和亚洲镰刀菌的方法,包括:使用引物通过PCR扩增基因的步骤;鉴定扩增基因的微卫星位点的步骤。诊断和区分红霉病原体种类的试剂盒包含引物和扩增试剂。

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