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MICROSATELLITE MARKER FOR IDENTIFYING SPECIES OF RED MOLD PATHOGEN AND METHOD FOR IDENTIFYING SPECIES OF RED MOLD PATHOGEN USING THE MARKER
MICROSATELLITE MARKER FOR IDENTIFYING SPECIES OF RED MOLD PATHOGEN AND METHOD FOR IDENTIFYING SPECIES OF RED MOLD PATHOGEN USING THE MARKER
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机译:用于鉴别红霉病原菌种的微卫星标记物和使用该标记物鉴别红霉病菌种的方法
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摘要
PURPOSE: A microsatellite marker for distinguishing species according to the type of toxin generated from red mold pathogen and a method for distinguishing species of pathogen using the same are provided to quickly and accurately distinguish main species and toxin by analyzing PCR using FgmsGT28. CONSTITUTION: A primer, FgmsGT28, of sequence numbers 1 and 2 is able to specifically amplify a microsatellite of red mold pathogen to distinguish Fusarium graminearum which produces deoxynivalenol(DON) and Fusarium asiaticum which produces nivalenol(NIV) by PCR(Polymerization Chain Reaction). A method for distinguishing F. graminearum and F. asiaticum comprises: a step of amplifying a gene using the primer by PCR; and a step of identifying a microsatellite site of the amplified gene. A kit for diagnosing and distinguishing species of red mold pathogen contains the primer and a reagent for amplification.
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