基于因子分析的定经汤主要药对菟丝子-柴胡调节卵巢早衰的作用机制

摘要

Objective To explore the biological mechanisms of Dingjing decoction herbs couples of Cuscuta Semen-Bupleuri Radix on the premature ovarian failure ( POF). Methods 120 SD rats were randomly divided into four groups including the normal group,the model group,the Dingjing Decoction group and the Cuscuta Semen-Bupleuri Radix group. The biomolecules associated with POF such as β-EP,IL-1,NOS, NO,ER, PR, GnRH, CRH, FSH, LH, E2, P, ACTH and CORT were detected. Regulation relations of biological molecules were explored based on factor analysis.Results The master regulatory factors regulated by Cuscuta Semen-Bupleuri Radix mainly contained the pituitary-ovarian axis four biomolecules, the pituitary-adrenal axis biomolecule CORT and part of hypothalamus biomolecules. GnRH was the common biomolecule in the hypothalamus part of the first and the other common factors regulated by Cuscuta Semen-Bupleuri Radix. Conclusion Cuscuta Semen-Bupleuri Radix can directly modulate pituitary-adrenal axis molecules,as well as indirectly modulate the pituitary-adrenal axis molecules through hypothalamus. GnRH plays a pivotal role in these factors.%目的：探讨定经汤主要药对菟丝子-柴胡调节卵巢早衰的作用机制。方法 SD大鼠随机分为4组：正常大鼠组、模型组、定经汤组和菟丝子-柴胡组；分别检测4组大鼠血清中与卵巢早衰相关的生物分子：β-EP、IL-1、NOS、NO、ER、PR、GnRH、CRH、FSH、LH、E2、P、ACTH和CORT；利用因子分析方法探究菟丝子-柴胡干预下各生物分子之间的相互调控关系。结果菟丝子-柴胡干预状态生殖内分泌网络主调控因子主要包括垂体-卵巢轴4个生物分子、垂体-肾上腺轴靶腺层生物分子CORT以及部分下丘脑生物分子；GnRH是菟丝子-柴胡干预状态主调控因子与其他公共因子共有的生物分子。结论菟丝子-柴胡一方面可以直接调节垂体-肾上腺轴生物分子，另一方面又可以通过下丘脑间接调节垂体-肾上腺轴生物分子；GnRH是各调控因子之间的枢纽生物分子。