烟饲五谷虫与常规饲养五谷虫油脂成分及体外药理活性比较研究

摘要

目的 研究烟饲五谷虫与常规饲养五谷虫的油脂成分组成及相对质量分数的差别,对比2种样品精油的体外抗氧化能力和体外抑菌活性.方法 采用CO2超临界萃取法分别提取五谷虫及烟饲五谷虫的油脂,粗油精制后,利用气质联用仪分析精油中脂肪酸组成和相对质量分数.采用DPPH法、ABTS法测定2种样品精油抗DPPH、ABTS自由基活性,牛津杯法测定2种精油对金黄色葡萄球菌和白色念珠菌、犬小孢子菌的抑制能力.结果 烟饲五谷虫与常规饲养五谷虫精油中化学成分组成基本一致,各成分相对质量分数稍有差别.2种精油体外均具有抗氧化活性,对比IC50值发现,烟饲五谷虫精油对于DPPH及ABTS 2种自由基的清除能力均强于常规饲养五谷虫精油.2种样品精油对金黄色葡萄球菌、白色念珠菌及犬小孢子菌均没有抑菌作用.结论 废弃烟叶饲养对于五谷虫油脂成分影响较小,且烟饲五谷虫精油的体外抗氧化活性增强,证实烟饲五谷虫的合理性,也为烟草废弃物的处理问题提供一定的理论基础.%Objective To compare the fatty acid composition,relative contents,free radicals scavenging capability and bacteriostatic ability of oil in conventional breeding maggots and tobacco feeding ones.Methods The oil of these two kinds of maggots were extracted by supercritical CO2 technology,and their fatty acid composition and relative content were analyzed by GC-MS.The antioxidant ability of the oil was evaluated by DPPH and ABTS assay while the antibacterial activities were investigated through Oxford cup method.Results Chemical compositions of these two kinds of oil were basically the same,while the components' relative content were slightly different.Antioxidant activities were demonstrated in both the oils.By the comparison of IC50 values,it was found that oil of tobacco feeding maggots displayed higher free radicals scavenging activity than that of conventional breeding ones.Both of them showed no bacteriostatic ability against Staphylococcus aureus,Candida albicans and Microsporum canis.Conclusion There is little difference in the fatty acid composition of conventional breeding maggots and tobacco feeding ones.The essential oil of tobacco feeding maggot enhanced antioxidant activity in vitro,which could confirm the rationality of the tobacco feeding maggot,and provide certain theoretical basis for handling problems of tobacco waste.