采用电化学法和光度法研究了手性乙二胺合钴(Ⅲ)配合物与DNA的作用.结果表明,两种旋光对映体与DNA发生作用时具有相同的电化学行为和相同的紫外可见光谱.平衡透析实验结果也证明,两种旋光对映体与DNA作用时,不存在立体选择性,即插入作用,这可能与配体分子结构的非平面性有关,两种对映体与DNA作用时,主要存在静电吸引作用.这项研究为设计新型的具有生物活性的小分子金属配合物提供了实验依据.%The binding of enantiomers of [Co(en)3]3+ (en=ethylenediamine) to calf thymus DNA is investigated by electrochemical and spectroscopic methods. Both isomers show absorption hypochromicity and the same CV behavior associated with binding to B-form DNA. CD spectra result shows no enantioselectivity for both isomers binding to DNA. All of this evidence suggests that both isomers bind to DNA by electrostatic mode.
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