壳聚糖-羟基磷灰石/Fe3O4磁性纳米载体的制备及性能∗

摘要

The nano-carries HAP/Fe3 O4 and HAP/Fe3 O4 was prepared by the method of acid-base neutraliza-tion.Superparamagnetic nano Fe3 O4 was added as nucleating agents.The microstructure,crystal phase,mag-netic and biological property were characterized by TEM,XRD,FT-IR,VSM,MTT.The results show that as-prepared carrier showed superparamagnetic,size of about 100 nm.HAP particles in bis-phase composite carrier was spherical,and short-rod like in three-phase composite carrier.Cell toxicity reaction in bis-phase composite carrier and three-phase composite carrier are 1 grade or 0 grade which has a role in promoting cell proliferation.%采用酸碱共滴定法,以超顺磁性的纳米Fe3 O4为形核剂,成功制备 HAP/Fe3 O4、CS-HAP/Fe3 O4磁性纳米载体。利用透射电镜(TEM)、X 射线衍射仪(XRD)、傅里叶红外光谱分析仪(FT-IR)、振动样品磁强计(VSM)和 MTT 法对样品的显微结构、物相、磁性能和生物学性能进行了表征和分析。结果表明,所制备载体表现为超顺磁性,尺寸约为100 nm,均为20 nm左右的羟基磷灰石颗粒包裹20 nm 左右的Fe3 O4颗粒而成。其中双相载体中的 HAP 颗粒呈球形,三相载体中的 H AP颗粒呈短棒状。双相载体为1级细胞毒性反应,三相载体为0级毒性反应,具有促细胞增殖作用。